Composite

Part:BBa_K5178016:Design

Designed by: Virginia iGEM 2024   Group: iGEM24_Virginia   (2024-10-02)


RAT_sequence_lac o_T7RNAP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2972
    Illegal NheI site found at 2976
    Illegal NheI site found at 2980
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2468
    Illegal XhoI site found at 787
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2901
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1390


Design Notes

The individual parts are designed to be joined together at the SacII restriction site following a digest and ligation.


Source

See individual parts for part sequence sources.

References